Egg leaf test. How to take a child for a kindergarten, pool, school

Tests for eggs of worms and the simplest microorganisms should be taken by each child before applying to a kindergarten, school, swimming pool or sports section.

Diagnosis of helminths in the gastrointestinal tract is carried out by microbiological examination of feces, taking swabs with an anal the perineal area, as well as the secretion of specific blood antibodies, which are produced by the immune system in response to parasitic invasion. In the absence of direct signs of infection with worms, they are limited to the delivery of feces and scrapings near the anus.

For what purpose is the analysis of feces for helminth eggs

Testing for the ovarian leaf is carried out in a planned manner, or to monitor the results of the therapeutic process. For example, if the child was previously diagnosed with helminths, and is currently being treated with antiparasitic drugs.

The analysis of feces for eggs of worms is used for the following purposes:

• confirmation of the presence or absence of whole helminths or their individual fragments, segments;
• determination of the species of already found worms parasitizing in the large or small intestine;
• prevention of helminthic infestations among children who attend kindergarten, schools, swimming pools or sports clubs;
• monitoring the success of the therapeutic process in a child who is undergoing complex treatment for helminthiasis;
• timely prevention of serious complications and negative consequences for the child's body, which is infected with parasitic life forms.

The main task of the examination for eggs of worms is the early diagnosis of helminthiasis, which will make it possible to form an effective course of treatment with minimal damage to the health of the child. This type of analysis is carried out in a public or private microbiological laboratory.

What exactly is determined in the analysis process?

It is simply necessary to take an egg leaf test for a child if he complains of constant nausea, itching in the anus, and also refuses to eat.

During the examination, the presence or absence of parasites in the gastrointestinal tract is determined:

• roundworms, which include the most common helminths in the form of ascaris and strongyloids;
• trematodes, as well as flatworms;
• tapeworms, which are among the most dangerous parasites;
• hookworms.

The number of helminth eggs in the feces depends on the severity of the parasitic invasion, the extent of the infection of the digestive system, as well as the child's immunity. In the process of carrying out this analysis, workers of the microbiological laboratory perform counting of the number of larvae found in the biological material.

Indications for mandatory testing for children

The study of feces for eggs of parasites is carried out in order to prevent helminthiasis, or after the detection of the primary signs of this disease.

Indications for mandatory testing for worms:

• nausea, which is constantly present, or arises under the influence of certain factors (for example, after eating, appears simultaneously with feeling of hunger, eating meat or sweet);
• prolonged diarrhea of ​​unknown etiology, when, in general, the child has no signs of infection of the gastrointestinal tract, or food poisoning;
• the release of vomit, along with which helminths exit through the esophagus (similar symptoms are typical when the digestive system is infected with tapeworms);
• deterioration in appetite, or a complete refusal to eat;
• itching in the anal area, which intensifies in the morning (at this time, fertilized female helminths go into the anus to lay eggs, which causes irritation of the peripheral receptors of the nerve systems);
• cramping pain in the navel, where the intestines are located, which can be filled with parasites;
• loose stools, accompanied by the release of blood, mucous contents of different color shades;
• visual detection in feces of whole helminths or their individual fragments, segments;
• previously diagnosed helminthiasis in the majority of close relatives who live with the child in the same family, have a common life, use the same kitchen utensils, bathroom (mother, father, brothers or sisters);
• fatigue, physical weakness and drowsiness, which is present even during those periods of time when the child slept, should be vigorous and active;
• involuntary salivation;
• various kinds of allergic reactions, which are manifested by skin rashes, spontaneous urticaria, itching and edema of epithelial tissues;
• swollen lymph nodes;
• the restless state of the child, when, due to severe itching in the perineal region, he constantly touches his genitals and the anal area;
• a febrile condition, which is accompanied by subfebrile body temperature (rises to + 37.0-37.5 degrees Celsius).

An egg leaf test should be passed to a child if he is applying for a kindergarten, school, swimming pool or sports section. These are mandatory preventive measures that are aimed at preventing an epidemiological outbreak of helminthiasis. In a closed children's collective, parasitic diseases spread very quickly.

Contraindications and restrictions

Microbiological examination of feces for the presence or absence of worm eggs has no direct medical contraindications for its conduct. If the general state of health of the child is satisfactory, he has primary signs of helminthiasis, he may self-defecate, then there are no restrictions on the selection of biological material for its further research.

Do you need preparation for the study?

Analyzing parasite eggs requires preliminary preparation.

In this case, it is necessary to follow the rules that will ensure the most objective result of microbiological examination of feces:

• visit a microbiological laboratory in advance, where you will need to take a sterile plastic container with a lid for collecting feces;
• 72 hours before the delivery of the biological material, rectal suppositories should not be introduced into the area of ​​the child's anus, regardless of which pharmacological group of drugs they belong to;
• children should not take medicines, the constituent substances of which, directly or indirectly, but still affect intestinal motility (for example, containing belladonna, or pilocarpine);
• 2 days before the examination, you should not use medications that change the color of the stool (preparations based on iron oxide, with the addition of bismuth, as well as barium sulfate);
• before the delivery of biological material, it is forbidden to carry out the hygiene of the genitals and skin in the anus, since the implementation of the data procedures can lead to biased results (after washing the skin and the anal area, fewer eggs will enter the fecal mass helminths);
• refuse to take laxatives, use medicinal oils for rectal administration;
• in the process of collecting feces, make sure that urine does not get into their composition.

It is recommended to take biological material in the morning during natural bowel movements. On the eve of the test, the child should eat as usual, eat the usual food without any special restrictions.

Feces collection begins immediately during the time when the child has a bowel movement, or after a bowel movement. In the second case, you will need to prepare a clean and sterilized pot in advance, from which the required amount of feces will be collected in a plastic container.

Reception and research of biomaterial

It is necessary to take an egg leaf test for a child if round or flat worms are present in his feces. The study of biological material for the presence or absence of helminth eggs is carried out in public or private microbiological laboratories. To carry out this diagnosis, microscopic testing is used. Identification and calculation of the numerical index of worm eggs is carried out manually by a laboratory assistant of the appropriate qualifications.

Before the delivery of the biological material to the sterile conditions of the microbiological laboratory, it is necessary to collect feces, adhering to the following algorithm of actions:

1. Place the child on a potty for a natural bowel movement without the use of enemas or rectal suppositories with laxative properties.
2. At the moment when the child has a bowel movement, it is necessary to ensure that no urine gets into the pot. To do this, under the organ of the genitourinary system, it is advisable to substitute another container that separates urine from feces.
3. Upon completion of defecation, the child should be lifted from the pot, and then hygiene of his genitals and skin in the anal area should be carried out.
4. With the help of a sterile plastic spoon that comes with the container, it is necessary to collect the feces.
5. The selection of biological material is carried out from different parts of the pot.
6. Particular attention is paid to softer stool fragments, as well as those that have signs of seals, mucous secretions, are distinguished by a lighter color shade.
7. The sterile shipping container is filled with biological material to 1/3 of its volume.
8. The container with feces is immediately closed with a tight lid to exclude further contact of its contents with environmental conditions.

Upon completion of the above manipulations, the container with biological material is delivered to the microbiological laboratory. Along with the feces, you must have a referral from your doctor or pediatrician.

For example, if the analysis for eggs of worms is handed over for preventive purposes for registration in a general educational institution. The maximum storage period for stool at home before it is transported to the laboratory is 12 hours. All this time, biological material can be in the refrigerator.

Acceptance of the transport container with feces is carried out by the laboratory assistant of the clinic where this research method is carried out. The transfer of biological material is carried out through a special window. The feces container is accompanied by accompanying documentation in the form of a referral from a doctor. After receiving samples for research, laboratory staff immediately begin the workflow.

This type of examination is carried out as follows:

1. The laboratory assistant performs the collection of feces from different parts of the container.
2. The biological material is applied in a thin layer onto a glass slide.
3. Stool samples are placed under the lens of an electron microscope.
4. The samples under study are stained with special chemical reagents, which make it possible to separate helminth eggs from the total mass of biological material.
5. If signs of helminthiasis are detected, the laboratory assistant counts the total number of worm eggs on a slide.

Based on the results of this diagnosis, one can conclude about the severity of helminthiasis, and also assume the number of parasites in the organs of the digestive system. In combination with a laboratory study of feces, a concomitant analysis for enterobiasis is mandatory.

This diagnosis is necessary to detect pinworm eggs, and its implementation is as follows:

1. The child's parents visit the microbiological laboratory, where they receive a microscope slide and special adhesive tape from the administrator.
2. Before taking biological samples in the evening and in the morning, do not wash the child.
3. In the morning on the day of diagnosis, it is necessary to wake the child up, and then lay him on one side, tucking his knees towards the chest.
4. Lower your panties.
5. Gently spread the baby's buttocks.
6. Using adhesive tape, collect a biological print from the skin located in the circumference of the anus.
7. Immediately adhere the adhesive tape to the surface of the sterile glass slide.

Samples of biological material are immediately delivered to the clinic at the place of examination. Along with the slide, you must have a referral from the attending physician.

Diagnosis for enterobiasis is carried out by a qualified laboratory worker in compliance with the following algorithm of actions:

1. The diagnostic center employee places a glass slide with adhesive tape attached under the viewing lens of the electron microscope.
2. The laboratory assistant stains samples of biological material using special chemical reagents.
3. In case of infection with pinworms, their eggs are found on the surface of the slide.
4. Helminth eggs are counted numerically.

Based on the results of the analysis for enterobiasis, an appropriate medical report is drawn up, which confirms or refutes the fact of parasitic invasion. Children whose digestive system is infected with pinworms are referred for further treatment to a pediatrician or parasitologist.

Typical Test Lead Time

It is extremely important to take an egg leaf test for a child if he has previously undergone complex treatment for helminthiasis. These actions are aimed at preventing the recurrence of the parasitic disease. Stool microscopy and enterobiasis analysis are performed within 24 hours.

Isolation of helminth eggs from the biological material does not require a lot of time, the use of sophisticated medical equipment or expensive consumables. State and private diagnostic centers with an outdated material and technical base, or a strong workload can perform this test for 2 days, but not longer than the specified period time.

Interpretation of the result

After the examination, the parents of the child receive a medical report, which indicates the results of microscopy of feces and diagnostics of the skin of the anal area for enterobiasis. The analysis is deciphered by a pediatrician or parasitologist who issued the appropriate direction.

The norm is the result in which the eggs of any type of worms are completely absent in the child's feces, as well as in the circumference of his anus. The presence of even 1 egg of parasites indicates the presence of helminthiasis.

Egg leaf test price

The average cost of microbiological examination of feces for the presence or absence of helminth eggs is 490 rubles. An analysis for enterobiasis is performed for 460 rubles. These are the average prices that are found in most privately owned clinics and microbiology laboratories. In public health institutions, tests for helminthiasis in children are carried out free of charge in the direction of a pediatrician or parasitologist.

Egg leaf tests are a microbiological study of feces, which is submitted by everyone a child before applying for a kindergarten, general education school, swimming pool or sports section (form 026y). The purpose of this diagnosis is the early detection of helminthiasis, as well as the prevention of complications of this disease.

Feces delivery for the possible presence of worm eggs is indicated for children of all age groups who have itching in the anal area, prolonged diarrhea of unknown etiology, there are spasmodic pains in the central part of the abdomen, nausea, vomiting, during bowel movements, round or flat worms of white colors. The test for helminthiasis is carried out by private and public clinics within 1-2 days.

Egg leaf test video

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